Activity of α-N-acetylgalactosaminidase (Nagalase) in serum or plasma is determined through a proprietary method consisting of a two step immunocapture assay.
In a first step Nagalase is captured on a solid phase coated with a α-N-acetylgalactosaminidase specific antibody able to capture up to 10 ng/ml of Nagalase from serum or plasma. After removal of unbound material the activity of immobilized Nagalase is measured by incubation with a specific α-N-acetylgalactosaminidase fluorogenic substrate. The resulting Nagalase enzymatic activity is expressed as nmol/min per milligram.
Normal values are below 0,95 nMol/min/m.
IMPORTANT: we are now accepting both plasma and serum samples for testing
Samples for Nagalase Activity Assay need to be collected as follows :
Testing on plasma: Whole blood must be collected in 1x9ml (or 2x4,5ml) EDTA tubes. Tubes must be centrifuged by nurse/physician within one hour at 3000 rpm for 10 minutes. Plasma has to be aliquoted in new, sterile tubes and frozen. Immediately freeze at least 3ml of plasma and ship to our lab on dry ice. Sample must be kept frozen at all time.
Testing on serum: Whole blood must be collected in serum tubes (like BD Vacutainer SST II Advanced tube, yellow cap). Tubes must be centrifuged (3000 rpm for 10 minutes) by nurse/physician AFTER one hour of cloting at room temperature. Serum has to be aliquoted in new, sterile tubes and frozen. Immediately freeze at least 3ml of serum and ship to our lab on dry ice. Sample must be kept frozen at all time. Centrifuged serum tube could be shipped on ambient temperature if its delivery is ensured within 24h after the blood sampling.